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Journal: BMC Biotechnology
Article Title: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen
doi: 10.1186/s12896-016-0249-x
Figure Lengend Snippet: Binding analysis of recombinant SNAP-TTC-BG647 on polyclonally activated CD19 + B cells. CD19 + B cells were isolated from leukocyte filters by density gradient centrifugation and magnetic cell separation with anti-CD19 beads. Isolated B cells were activated by adding 2.5 μg/ml CpG and 50 ng/ml IL-21 to the cell culture medium. On day 4 of cultivation, the cells were analyzed by flow cytometry. Surface a and intracellular b staining with the recombinant SNAP-TTC-BG 647 (1:25) was performed
Article Snippet: The staining was performed with the following monoclonal antibodies against the
Techniques: Binding Assay, Recombinant, Isolation, Gradient Centrifugation, Magnetic Cell Separation, Cell Culture, Flow Cytometry, Staining